Screening of 258 Pesticide Residues in Silage Using Modified QuEChERS with Liquid- and Gas Chromatography-Quadrupole/Orbitrap Mass Spectrometry

A method for the simultaneous screening of 258 pesticide residues in silage using modified QuEChERS combined with liquid chromatography (LC)- and gas (GC)- quadrupole-Orbitrap mass spectrometry (Q-Orbitrap/MS) has been developed. After hydration, was homogenized a 1% acetic acid–acetonitrile solution, extract purified C18, PSA, anhydrous magnesium sulfate. Finally, sample detected LC/GC-Q-Orbitrap/MS, quantified an external standard method. The results showed that pesticides had excellent linear relationship range 0.1–50 ?g L?1, coefficients determination (R2) were more than 0.99. detection limit (SDL) 0.5–50 kg?1, quantitation (LOQ) 1–50 kg?1. accuracy precision verified at spiked levels 1-, 2- 10-times LOQ, recovery 66.5–119.8%, relative deviations (RSDs) less 20% (n = 6). This simple, rapid, reliable, could be applied to screen quantify multi-pesticide silage.